. Describe briefly the followings

(i) DNA polymerase enzyme

(ii) DNA ligase enzyme


Best Answer

(i) DNA polymerase enzyme: It is used for producing copies of DNA through PCR technique or replication of DNA under usual condition (DNA directed DNA synthesis). It was first discovered by Kornberg.

(ii) DNA ligase enzyme: A type of enzyme used to seal the cut ends of DNA fragments. It is a type of molecular adhesive used during joining of desired gene with suitable vector DNA. DNA ligase was first discovered by Dr. Khorana from T4-bacteriophages and thus named as T4-ligase.


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