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Viroids, Definition, Types, Characteristics and Diseases

Viroids, This article aims to provide an overview of viroids, including their definition, types, characteristics, structure, diseases, and related MCQs with answers.
authorImageKrati Saraswat4 Jun, 2025
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Viroids

Viroids are unique infectious agents that primarily infect plants, causing various diseases. Unlike viruses, viroids lack a protective protein coat and contain only a short strand of circular RNA. Understanding viroids is crucial for NEET biol ogy , especially when studying plant pathology and the diversity of infectious agents.

Definition of Viroids

Viroids are infectious RNA molecules, smaller than viruses , that cause diseases in plants. They are among the smallest known agents of infection and consist solely of a single, circular RNA strand without any protein coating. Viroids do not infect animals or humans and are highly specific to plant hosts.

Discovery of Viroids

The concept of viroids was first introduced in 1971 when Theodor O. Diener discovered the Potato Spindle Tuber Viroid (PSTVd), which affects potatoes. This discovery demonstrated that infectious agents could exist without the protein structure that characterizes typical viruses.

Structure of Viroids

Viroids differ significantly from viruses in their structure. Unlike viruses, viroids are composed solely of short strands of circular, single-stranded RNA, without any protective protein coat. This simple structure makes them some of the smallest known infectious agents that cause disease. Viroids primarily infect plants , causing serious agricultural losses each year. Some commonly affected crops include potatoes, tomatoes, cucumbers, and coconut palms. Infected plants provide the cellular machinery needed for viroid replication within organelles like the nucleus or chloroplasts. Lacking protein-coding capabilities, viroids replicate through RNA–RNA transcription and infect plant tissues via cell wall damage, leading to severe crop impact.

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Characteristics of Viroids

The unique features of viroids make them distinct from other pathogens. Here are some key characteristics:
  • Viroids consist solely of RNA.
  • They are extremely small and infect only plants.
  • Viroids are among the smallest known infectious agents.
  • They have a low molecular weight and a unique RNA structure.
  • Viroids replicate within the host cell, causing changes that can eventually kill the plant.
  • They are classified into two families: Pospiviroidae, which replicate in the nucleus, and Avsunviroidae, which replicate in the chloroplast.
  • Viroids can move within plant cells through structures called plasmodesmata and travel long distances through the plant's phloem.

Types of Viroids

Viroids are classified into two primary families: Pospiviroidae and Avsunviroidae:
  • Pospiviroidae : The Pospiviroidae family includes the type species Potato Spindle Tuber Viroid (PSTVd) and comprises five genera with over 24 species. This family is characterized by members that possess five major domains in their secondary structures and undergo asymmetric rolling-circle replication in the nucleus, typically lacking ribozyme activity. The Pospiviroidae family can infect a wider range of hosts , including both monocot and dicot plants . This classification highlights the diverse nature and infection patterns of viroids across different plant species.
  • Avsunviroidae : The Avsunviroidae family features the type species Avocado Sunblotch Viroid (ASBVd) and consists of two genera with three total species. These viroids are known for their branched secondary structures and utilize a chloroplast-based symmetric rolling-circle replication mechanism, which includes ribozyme activity. Viroids from the Avsunviroidae family are restricted to infecting dicotyledonous plants, which can be either herbaceous or woody.

Viroid Diseases

Viroids cause several plant diseases, including cucumber pale fruit, citrus exocortis, and chrysanthemum stunt. These infections spread through cuttings, tubers, and other seed propagation methods, as well as through contaminated tools. Viroid-like particles also lead to hepatitis D in humans. Symptoms of viroid infection in plants include stem necrosis, stunted growth, distorted leaves and fruits, and eventually plant death. Viroids infect various plants, including coconut and apple trees. The potato spindle tuber viroid (PSTVd) results in elongated and cracked tubers, causing significant crop losses. Examples of Viroid Disease : Citrus Gummy Bark Viroid, Grapevine Viroid, Dapple Peach Fruit Disease Viroid, Potato Spindle Tuber Viroid (affecting potatoes), Cucumber Pale Fruit Viroid, Dapple Plum and Peach Fruit Disease Viroid.

Differences Between Viroids and Viruses

The table below compares viroids and viruses, focusing on their structure, host types, and unique features. This distinction is crucial for understanding how these infectious agents impact their respective hosts differently.
Feature Viroids Viruses
Genetic Material RNA only, free of protein coat DNA or RNA, enclosed in a protein coat (capsid)
Size Smaller than viruses Generally larger than viroids
Infect Host Types Primarily infect plants Infect various types of organisms, such as animals, plants, and bacteria.
Protein Coat Absent Present, known as the capsid
Replication Can only replicate within a plant host cell Requires a host cell to replicate, can infect various cell types
Structure RNA molecule without any protein covering Nucleoprotein particle, consisting of genetic material and a capsid
Infectious Nature The smallest infectious agents that primarily attack plants Tiny infectious agents that infect various types of living cells

MCQs of Viroids

Q1. Which disease is caused by viroids?

  1. Leaf curling disease
  2. Potato spindle tuber disease
  3. Dwarfing disease
  4. All of these

Q2. In 1971 T.O. Diener discovered a new infectious agent that was smaller than viruses;

(i) it causes potato spindle tuber disease.

(ii) it is free RNA.

(iii) molecular wt. of RNA is low.

The above statements are assigned to;

  1. viruses.
  2. viroids.
  3. virulent.
  4. mycoplasma.

Q3.. Viroids differ from viruses in having;

  1. DNA molecules with protein coat.
  2. DNA molecules without protein coat.
  3. RNA molecules with protein coat.
  4. RNA molecules without protein coat.

Answers of MCQs of Viroids

Ans1. Potato spindle tuber disease,
 Ans2. Viroids,
 Ans3. RNA molecules without protein coat.
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Viroids FAQs

Q. Who discovered viroids?

Ans. Viroids were discovered by Theodor O. Diener in 1971. He identified them as the causal agent of the potato spindle tuber disease, marking the first recognition of viroids as distinct infectious agents.

Q. What is a viroid?

Ans. A viroid is a small, infectious particle made of circular RNA that lacks a protective protein coat. Viroids are the smallest known agents of infectious disease and are unique in that they infect plants exclusively, disrupting normal cellular functions.

Q. Viroids differ from viruses in having?

Ans. Viroids differ from viruses in having only RNA without any protein coat. This simpler structure means viroids are typically smaller and only infect plants, unlike viruses that can infect a broader range of hosts.

Q. What is the structure of a viroid?

Ans. Viroids have a very simple structure, consisting of a short, circular strand of RNA without a surrounding protein coat. This lack of a protein shell distinguishes them from viruses and limits their ability to infect beyond plant cells.

Q. What is the difference between viruses and viroids?

Ans. The main difference lies in their structure and host range. Viruses contain nucleic acids (either DNA or RNA) within a protein coat, allowing them to infect animals, plants, and bacteria. Viroids, on the other hand, contain only RNA, lack a protein coat, and exclusively infect plants.

Q. What is an example of a viroid?

Ans. An example of a viroid is the Potato Spindle Tuber Viroid (PSTVd), which causes the spindle tuber disease in potatoes, leading to stunted growth and poor crop yield.
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