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BSc Nursing 2nd Semester Applied Biochemistry Syllabus: Unit-Wise Syllabus, Marks Distribution & Preparation Tips

The BSc Nursing 2nd Semester Applied Biochemistry Syllabus introduces students to metabolism, enzymes, acid-base balance, organ function tests, immunochemistry, and their clinical applications. The subject carries 35 marks, including 10 marks for internal assessment and 25 marks for the university examination. Understanding the BSc Nursing 2nd Semester Applied Biochemistry unit wise syllabus and following a smart preparation strategy can help students score well in the semester exam.
authorImageAnanya Gupta18 Jul, 2026
BSc Nursing 2nd Semester Applied Biochemistry Syllabus: Unit-Wise Syllabus, Marks Distribution & Preparation Tips

The BSc Nursing 2nd Semester Applied Biochemistry Syllabus is designed according to the revised Indian Nursing Council (INC) competency-based curriculum. It helps nursing students understand how biochemical processes affect the human body and their importance in patient care.

The subject covers carbohydrate, protein, and lipid metabolism, enzyme functions, acid-base balance, organ function tests, and immunochemistry. These concepts enable nursing students to interpret laboratory reports, identify metabolic disorders, and provide better clinical care.

BSc Nursing 2nd Semester Applied Biochemistry Marks Distribution

The BSc Nursing 2nd Semester Applied Biochemistry marks distribution is combined with Applied Nutrition & Dietetics in one theory paper. However, the marks for both subjects are evaluated separately.

BSc Nursing 2nd Semester Applied Biochemistry Marks Distribution

Assessment

Applied Biochemistry

Applied Nutrition & Dietetics

Total

Internal Assessment

10 Marks

15 Marks

25 Marks

University Theory Exam

25 Marks

50 Marks

75 Marks

Total

35 Marks

65 Marks

100 Marks

Passing Criteria: Students must score at least 50% separately in the internal assessment and university theory examination to pass the semester.

BSc Nursing 2nd Semester Applied Biochemistry Unit Wise Syllabus

The syllabus is divided into eight units that gradually build your understanding of clinical biochemistry.

Unit I: Carbohydrate Metabolism and Alterations

Topics covered include:

  • Digestion and absorption of carbohydrates

  • Glycolysis

  • Glycogenesis

  • Gluconeogenesis

  • TCA (Krebs) Cycle

  • Blood glucose regulation

  • Diabetes Mellitus and its biochemical changes

Unit II: Lipid Metabolism and Alterations

Students will study:

  • Fatty acids (MUFA, PUFA and Trans fats)

  • Digestion and absorption of lipids

  • Lipid metabolism

  • Ketone bodies

  • Lipoproteins

  • Cholesterol metabolism

  • Atherosclerosis

  • Lipid profile interpretation

Unit III: Protein and Amino Acid Metabolism

Important topics include:

  • Classification of amino acids

  • Protein digestion and absorption

  • Plasma proteins

  • Nitrogen metabolism

  • Phenylketonuria

  • Alkaptonuria

  • Proteinuria

  • Hypoproteinemia

  • Hypergammaglobulinemia

Unit IV: Clinical Enzymology

This unit covers:

  • Enzymes and co-enzymes

  • Classification of enzymes

  • Clinical importance of enzymes

  • Cardiac enzyme markers

  • Liver enzyme markers

  • Bone enzyme markers

  • Amylase and Lipase

Unit V: Acid-Base Balance and Imbalances

Students learn about:

  • Body fluid compartments

  • Buffer systems

  • Respiratory regulation

  • Renal regulation

  • Arterial Blood Gas (ABG) analysis

  • Metabolic acidosis

  • Metabolic alkalosis

  • Respiratory acidosis

  • Respiratory alkalosis

Unit VI: Haemoglobin Metabolism

Topics include:

  • Heme synthesis

  • Heme degradation

  • Bilirubin metabolism

  • Direct and indirect bilirubin

  • Jaundice

Unit VII: Organ Function Tests

Students study important diagnostic tests such as:

Renal Function Tests (RFT)

  • Blood Urea Nitrogen (BUN)

  • Serum Creatinine

  • Uric Acid

Liver Function Tests (LFT)

  • Bilirubin

  • Albumin

  • Globulin

  • AST

  • ALT

Thyroid Function Tests (TFT)

  • T3

  • T4

  • TSH

Unit VIII: Immunochemistry

This unit focuses on:

  • Immunoglobulins (IgG, IgA, IgM, IgD and IgE)

  • Antigen-antibody reactions

  • ELISA

  • Western Blot

  • Clinical applications of serological tests

How to Prepare for BSc Nursing 2nd Semester Applied Biochemistry

If you are wondering how to prepare for BSc Nursing 2nd Semester Applied Biochemistry, follow these practical tips.

1. Understand Clinical Applications

Rather than memorising biochemical pathways, understand how they relate to diseases such as diabetes, jaundice, kidney disorders, and acid-base imbalance.

2. Learn Important Laboratory Values

Keep a notebook of normal laboratory reference values, including:

  • Fasting Blood Sugar

  • Serum Creatinine

  • Blood Urea Nitrogen (BUN)

  • Serum Bilirubin

  • Blood pH

These values are frequently tested in university and nursing competitive exams.

3. Practice Metabolic Cycles

Focus on understanding:

  • Glycolysis

  • TCA Cycle

  • Glycogenesis

  • Gluconeogenesis

You don't need to memorise every chemical reaction, but you should understand their clinical significance.

4. Master ABG Interpretation

Practice identifying:

  • Metabolic Acidosis

  • Metabolic Alkalosis

  • Respiratory Acidosis

  • Respiratory Alkalosis

ABG questions are common in nursing examinations.

5. Prepare Short Notes

Most university questions ask students to write short notes. Regularly revise topics such as:

  • Lipid Profile

  • Plasma Proteins

  • Liver Function Tests

  • Cardiac Enzymes

  • Bilirubin

  • ELISA

  • Immunoglobulins

6. Revise with Previous Year Questions

Solve previous university papers to understand the pattern of short-answer and long-answer questions.

BSc Nursing 2nd Semester Applied Biochemistry Syllabus FAQs

How to prepare for BSc Nursing 2nd Semester Applied Biochemistry?

To prepare effectively, understand metabolic pathways, learn important laboratory values, practise ABG interpretation, revise enzyme functions, prepare short notes, and solve previous years' question papers regularly.

Is Applied Biochemistry difficult in BSc Nursing 2nd Semester?

Applied Biochemistry can seem challenging initially because it involves metabolism and laboratory concepts. However, focusing on clinical applications, regular revision, and concept-based learning makes the subject much easier to understand and score well in.

What are the most important topics in the BSc Nursing 2nd Semester Applied Biochemistry Syllabus?

Some of the most important topics include carbohydrate metabolism, lipid metabolism, acid-base balance, organ function tests (LFT, RFT, TFT), enzyme markers, bilirubin metabolism, diabetes mellitus, and immunochemistry.

Is the BSc Nursing 2nd Semester Applied Biochemistry syllabus important for competitive nursing exams?

Yes. The syllabus forms the foundation for nursing entrance and recruitment exams such as AIIMS NORCET, ESIC Nursing Officer, DSSSB Nursing Officer, PGIMER, and other state nursing examinations, where questions related to metabolism, enzymes, laboratory values, and acid-base balance are frequently asked.
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