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Hypotonic Solution - Definition, Examples, and Importance

A hypotonic solution is when water flows into a lower solute concentration than the cell. Hypotonic solution, definition, swelling and shrinking phenomenon examples, and importance are given below.
authorImageKrati Saraswat12 Aug, 2025
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Hypotonic Solution

Hypotonic Solution: Tonicity is the measurement of the effective osmotic pressure gradient. It involves comparing the water potential of two solutions separated by a partially permeable cell membrane. Tonicity is determined by the relative concentration of solutes that the membrane allows to pass through, which affects the extent and direction of osmotic flow.

A solution can be classified as hypotonic, hypertonic, or isotonic compared to another solution. A hypotonic solution has a lower solute concentration than the other solution across a semipermeable membrane. This causes water to move into the cell, leading to swelling or even bursting. As a result, the osmotic pressure in a hypotonic solution is lower than in other solutions. Information about hypertonic solutions in NEET Biology Notes can be found in the article below.

Hypotonic Solution Definition

The capacity of an extracellular solution to influence water movement into or out of a cell via osmosis is termed 'tonicity'. Tonicity correlates with the solution's osmolarity. Examination of a hypotonic solution can enhance comprehension of this concept. Osmolarity denotes the collective concentration of solutes within a solution. Lower osmolarity signifies fewer solute particles per litre, while higher osmolarity indicates a greater number of solute particles per litre. A hypotonic solution has a lesser solute concentration compared to another solution. The classification of a solution as hypotonic, isotonic, or hypertonic depends on its comparison to another solution. Understanding tonicity and osmolarity is vital for cell description. Osmolarity, measuring solute concentration per litre across various solutions, elucidates the formation of water and solute gradients. For NEET exam preparation, refer to NEET Biology MCQ and NEET Previous Year Question papers related to this topic.

Hypertonic Solution diagram

Mammary Glands

Hypotonic Solution Examples

Hypotonic solutions are those with a lower solute concentration compared to a reference solution, often a cell or blood plasma. This means that there is a higher proportion of water to solutes in a hypotonic solution. Here are some common hypotonic solution examples:
  • Distilled water: Distilled water has minimal solutes, making it hypotonic to most cells.
  • Plain, unsweetened tea or coffee: These beverages have some dissolved substances, but much less than blood or cells.
  • Coconut water: While it contains some electrolytes, coconut water is generally considered hypotonic to blood plasma.
  • Certain sports drinks: Some sports drinks are designed to be hypotonic to assist in rehydration after exercise. However, it's important to check the label, as some sports drinks can be hypertonic.
It's important to remember that the tonicity of a solution can vary depending on the reference solution used. For example, a 5% dextrose solution (sugar water) is hypotonic to blood plasma but hypertonic to cells. This is because the dextrose cannot freely pass through the cell membrane, contributing to the solution's solute concentration outside the cell.

Okazaki Fragments

Hypotonic Solution Swell or Shrink

Hypotonic solutions contain a lower concentration of solutes, such as salts and sugars, compared to the solute concentration inside the cell. This difference in concentration causes water to move into the cell through osmosis, leading to the cell swelling. Osmosis is the process of water moving through a semipermeable membrane from an area of low solute concentration (hypotonic solution) to an area of high solute concentration (the cell) to equalize concentrations on both sides. This movement of water can cause cells to shrink or swell depending on the surrounding solution. Visualize a cell as a small balloon filled with sugary water (representing cytoplasm) and surrounded by a thin, elastic membrane (plasma membrane). The membrane acts as a gatekeeper, allowing some substances to pass through while keeping others out. In a hypotonic solution, which is like submerging our balloon in a large tub of plain water, the solute concentration outside the cell is lower than inside the cell. This creates a situation where water, being the key player, moves as follows:
  1. Osmosis Takes Place: Water naturally moves from an area of low solute concentration (outside the cell) to an area of high solute concentration (inside the cell) to balance the concentrations.
  2. Water Influx: Because there's more sugary water inside the cell than outside, water molecules flow into the cell through the semipermeable membrane.
  3. Cell Swells: As water enters, the cell swells and expands. The more water that enters, the larger the cell becomes.
Red blood cells are a prime example of how hypotonic solutions impact cells. Think of being caught in a sudden downpour; rainwater, being hypotonic compared to the concentrated salt solution inside red blood cells, can cause them to swell and burst (hemolysis) if exposed for too long, such as in a deep wound.

lymphocytes

Hypotonic Solution Importance

Hypotonic solutions, with their lower solute concentration compared to cellular environments, play a pivotal role in biological processes. These solutions facilitate water influx into cells through osmosis, serving several key functions:
  1. Hydration: Hypotonic solutions are crucial for rehydrating individuals experiencing dehydration from conditions such as diarrhoea, vomiting, or burns. The influx of water into cells restores proper cellular function.
  2. Drug Delivery: These solutions are utilised to deliver medications or nutrients directly into cells, aided by osmotic pressure gradients.
  3. Cellular Swelling: Controlled cell swelling can be beneficial in certain medical conditions. For instance, hypotonic solutions are used to treat glaucoma by increasing fluid pressure within the eye, aiding in lens repositioning and improved drainage.
Apart from their biological role, hypotonic solutions have applications in other domains:
  1. Food Preservation: They are used to shrink bacterial cells, prolonging the shelf life of food products.
  2. Agriculture: Hypotonic solutions promote seed germination by facilitating water uptake, crucial for initial plant growth stages.

Maintaining Cellular Equilibrium

While hypotonic solutions offer various benefits, maintaining solute concentration balance is critical. Excessive water influx can cause cell swelling and potential bursting, while excessive efflux can lead to cell shrinkage and dehydration, both detrimental to cell health. In summary, hypotonic solutions are integral to biological processes and have significant medical and industrial applications. Understanding their impact on cells and the importance of cellular balance is crucial for their effective and safe use.

Gametes Types

Hypotonic Solution and Hypertonic Solution

Hypotonic and hypertonic solutions are important concepts in biology, particularly in understanding how cells interact with their environment. These solutions have different effects on cells due to their differing concentrations of solutes. Here's a comparison table to highlight the key differences:
Hypotonic Solution vs. Hypertonic Solution
Aspect Hypotonic Solution Hypertonic Solution
Definition Lower concentration of solutes than the cell Higher concentration of solutes than the cell
Effect on Cells Causes cells to swell (cytolysis) Causes cells to shrink (plasmolysis)
Osmotic Pressure Lower than the cell Higher than the cell
Example Pure water Saline solution
Biological Importance Prevents cells from drying out; used in cell culture Preserves food by drawing out water from bacteria
In summary, hypotonic solutions have a lower concentration of solutes compared to the cell, leading to water entering the cell and causing it to swell. On the other hand, hypertonic solutions have a higher concentration of solutes, causing water to leave the cell and leading to cell shrinkage. Physics Wallah offers superior NEET Online Coaching , making learning easy and effective for students pursuing the NEET exam. PW online coaching features expert teachers who simplify complex concepts, enhancing the students' chances of success in their studies.
NEET Exam Important Links
NEET Syllabus NEET Biology Diagrams
NEET Biology Notes NEET Biology Chapter wise Weightage

Hypotonic Solution FAQs

What are Hypotonic and Hypertonic Solutions?

A hypertonic solution contains a higher concentration of solute than another solution, causing water to move into it. Conversely, a hypotonic solution contains a lower concentration of solute, causing water to move out of it.

What is a Hypertonic Solution?

A hypertonic solution is a type of solution with a higher concentration of solutes outside a cell than inside it.

What is the Difference Between Hypotonic and Isotonic Solutions?

The key difference lies in their osmotic pressures. Isotonic solutions have equal osmotic pressures, while hypotonic solutions have lower osmotic pressures, and hypertonic solutions have higher osmotic pressures.

What is an example of a Hypertonic Solution?

An example is the interior of a red blood cell compared to the solute concentration of fresh water.

Why is Hypertonic Solution Used?

Clinicians use hypertonic fluids to increase intravascular fluid volume and in the treatment of hyponatremia. Hypertonic saline and mannitol are also used to reduce intracranial pressure.

What is a Hypotonic Solution in Class 12?

A hypotonic solution has a lower concentration of solute inside the cell than outside the cell.

What is a hypotonic solution?

A hypotonic solution is one with a lower solute concentration than the cell, causing water to enter and swell the cell. This concept is key in the NEET Biology Syllabus and appears in NEET Biology MCQ and NEET Previous Year Question papers.
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