Physics Wallah

Difference Between Mitochondria and Chloroplast, Structure and Functions

Difference Between Mitochondria and Chloroplast, mitochondria produce cellular energy, while chloroplasts in plants and algae capture light energy for photosynthesis. Mitochondria and chloroplasts are plant cell organelles.
authorImageKrati Saraswat23 May, 2025
Share

Share

Difference Between Mitochondria and Chloroplast

Difference Between Mitochondria and Chloroplast:  A eukaryotic cell has only three organelles with a double-layered structure: the nucleus, mitochondria, and chloroplast. While these organelles share structural similarities, their primary difference is their location. Mitochondria are found inside cells in humans and plants, converting stored energy in molecules or other fuel sources into a form the cell can use.

On the other hand, chloroplasts are organelles present in the cells of plants and algae. They absorb light energy and store it as fuel molecules within the plant's tissues. The following article details the difference between mitochondria and chloroplasts in eukaryotic cells.
NEET Biology Syllabus NEET Biology Diagrams
NEET Biology MCQ NEET Biology Chapter wise Weightage
NEET Biology Notes NEET Previous Year Question papers

Difference Between Mitochondria and Chloroplast Overview

Mitochondria and chloroplasts are both cellular organelles present in plant cells. However, while mitochondria are found in plant and animal cells, chloroplasts are exclusive to plant cells. Mitochondria are the cell's energy generators, transforming oxygen and nutrients into ATP. Conversely, chloroplasts are responsible for facilitating photosynthesis in plant cells. Mitochondria are tiny organelles found throughout the cell and widely regarded as the cell's energy source.
Specific cells, such as muscle cells, have many mitochondria to meet their high energy demands. These organelles are made up of two membranes: an outer membrane that surrounds the organelle and an inner membrane that folds into numerous cristae.  Chloroplasts, on the other hand, are only found in plant cells and algae and serve as photosynthesis sites.
Chloroplasts are located in the cell's cytosol and have their DNA, allowing them to replicate independently of the cell. They collect light energy and convert it to chemical energy. The following article explains the difference between mitochondria and chloroplasts in greater detail.

Difference Between Mitochondria and Chloroplast

Mitochondria and chloroplasts are eukaryotic cell organelles involved in energy conversion and metabolism. Chloroplasts participate in photosynthesis, which converts light energy into chemical energy, specifically glucose. Meanwhile, mitochondria generate energy in the form of ATP via oxidative phosphorylation. The table below outlines the specific difference between mitochondria and chloroplasts:

Difference Between Mitochondria and Chloroplast

Characteristic Mitochondria Chloroplasts
Location Found in almost every eukaryotic cell, including animals and plants Found only in some protists, algae, and plant cells
Size and Shape Usually 1 to 10 micrometers in length, sausage-shaped Typically 2 to 10 micrometers long, disc or oval-shaped
Membrane Structure Outer and inner membrane, separated by an intermembrane space Thylakoid membrane, inner membrane, and outer membrane
Replication Binary fission replication Binary fission or budding replication
Pigments Doesn't contain any pigments Contains pigments such as chlorophyll
DNA Contains its own circular DNA, similar to bacterial DNA Contains its own circular DNA, similar to bacterial DNA
Function Uses oxidative phosphorylation to produce ATP Utilizes photosynthesis to convert light energy to chemical energy
Endosymbiotic Origin Likely descended from an ancestral eukaryotic cell that engulfed a free-living bacterium Likely descended from an ancestral eukaryotic cell that engulfed a free-living photosynthetic cyanobacterium
Oxygen Dependence Utilizes oxidative phosphorylation, requiring oxygen for ATP production Does not need oxygen, but some processes can use it
Enzymes and Pathways Involved in the electron transport chain, citric acid cycle, and beta-oxidation Involved in light-dependent and light-independent reactions of photosynthesis

Mitochondria

Mitochondria in almost all eukaryotic cells serve as the cell's powerhouses by producing ATP, the energy currency.  In addition to energy production, mitochondria contribute to cellular activities such as heat production, calcium storage for signalling, and cell growth and differentiation. A human cell typically contains 100-1000 mitochondria, each of which is essential for cell function. Inherited maternally, their DNA differs from the cell's nuclear DNA.
The number of mitochondria varies among organisms and cell types, with cells containing multiple mitochondria based on their functions.  Mitochondria, with their double-layered membrane, vary in shape and size and comprise about a quarter of a cell's volume. Initially thought of as isolated entities, they are now understood to link to form dynamic networks known as a reticulum, consisting of four main compartments. Mitochondria, vital for aerobic metabolism, substantially contribute to cellular energy production via oxidative phosphorylation, which accounts for about 90% of ATP generation.
They maintain, replicate, and transcribe their circular DNA while engaging in various metabolic processes, including iron-sulfur cluster synthesis. 

Explore -

Chloroplast

Chloroplasts in plants and green algae are specialized organelles housing chlorophyll for solar energy absorption. Their green colour stems from the pigments chlorophyll a and chlorophyll b. Functioning as semi-autonomous entities, chloroplasts possess their DNA and consist of a double-layered structure. Variations in chloroplast number and size occur in different cell types, with shade-grown plants exhibiting larger chloroplasts than those in intense light. Chloroplasts vary, from plate-shaped in Chlorella to spherical or ovoid in higher plants. 
They comprise an outer membrane, intermembrane space, inner membrane, stroma, and thylakoids. Thylakoids, arranged in stacks called Grana, are vital for harnessing solar energy in photosynthesis.  Photosynthesis occurs primarily in chloroplasts, converting solar energy into chemical energy.  In addition to photosynthesis, they contribute to synthesising fatty acids, amino acids, and membrane lipids. Plants, unlike animals, lack specialized immune cells, and all plant cells play an active role in the plant's defence mechanisms.
NEET Biology Difference Between Important Links
Difference Between Frog And Toad Difference between Homozygous and Heterozygous
Difference Between Small Intestine and Large Intestine Difference Between Carpel and Pistil
Difference Between Monohybrid And Dihybrid Difference Between Sperm and Ovum
Difference Between Cytoplasm and Protoplasm Difference Between Erosion and Weathering
Difference Between Endotoxins And Exotoxins Difference Between Right And Left Lung
Difference Between Euchromatin And Heterochromatin Difference between Gene and Chromosome
Difference between Open and Closed Circulatory System Difference Between Transpiration And Guttation
Difference between Voluntary and Involuntary Muscles Difference Between Mixed Cropping and Intercropping
Difference Between Gene and Allele Difference Between Axon and Dendrites
Difference Between Humoral and Cell-Mediated Immunity Difference Between Environment and Ecosystem
Difference Between Simple And Complex Tissue Difference between Renewable and Non-renewable Resources
Difference Between Vaccination And Immunization Difference Between Aestivation And Hibernation
Difference between Heart Attack and Cardiac Arrest Difference Between Cardiac Muscle And Skeletal Muscle
Difference Between Afforestation and Deforestation Difference Between Mitochondria And Chloroplast
Difference Between Egestion And Excretion Difference between Cell and Tissue
Difference Between Locomotion and Movement Difference between Monocot and Dicot Stem
Difference Between Lytic and Lysogenic Cycle Difference Between Glycolysis and Krebs Cycle
Difference Between Monocotyledon and Dicotyledon Difference between MCB and MCCB
Difference Between Nucleus and Nucleoid Difference between Solar and Lunar Eclipse
Difference Between Pharynx And Larynx Differences Between Magma and Lava

Difference Between Mitochondria and Chloroplast FAQs

What is the difference between mitochondria and chloroplast?

In plant cells, chloroplasts convert light energy into chemical energy, while mitochondria utilize this chemical energy to produce ATP. To achieve optimal carbon fixation and promote plant growth, these two organelles, each responsible for energy transformation, must coordinate their actions effectively.

Do chloroplasts possess DNA?

Chloroplasts, belonging to the plastid family, and mitochondria play central roles in ecosystem and biosphere energy cycles. Both organelles contain DNA organized into nucleoids, encoding crucial genes essential for photosynthetic and respiratory energy production.

What distinguishes mitochondria from mitochondrion?

The sole distinction between mitochondria and mitochondrion lies in grammatical number; mitochondrion is singular, and mitochondria is its plural form. Within a cell, mitochondria, responsible for nutrient breakdown into usable energy molecules, play a vital role in cellular energy processes.

Do chloroplasts and mitochondria share similar DNA?

The resemblance between the DNA of mitochondria, chloroplasts, and bacteria supports the endosymbiont theory. This theory proposes that mitochondria and chloroplasts originated as independent prokaryotic cells, highlighting a significant connection between their genetic material and that of bacteria.

Are mitochondria exclusive to females?

Mitochondrial DNA (mtDNA) inheritance occurs maternally, passed from mother to child. While both sons and daughters inherit mtDNA, only daughters transmit it to their offspring. Consequently, both men and women can undergo mtDNA tests, as they inherit their mother's mtDNA.
Join 15 Million students on the app today!
Point IconLive & recorded classes available at ease
Point IconDashboard for progress tracking
Point IconMillions of practice questions at your fingertips
Download ButtonDownload Button
Banner Image
Banner Image
Free Learning Resources
Know about Physics Wallah
Physics Wallah is an Indian edtech platform that provides accessible & comprehensive learning experiences to students from Class 6th to postgraduate level. We also provide extensive NCERT solutions, sample paper, NEET, JEE Mains, BITSAT previous year papers & more such resources to students. Physics Wallah also caters to over 3.5 million registered students and over 78 lakh+ Youtube subscribers with 4.8 rating on its app.
We Stand Out because
We provide students with intensive courses with India’s qualified & experienced faculties & mentors. PW strives to make the learning experience comprehensive and accessible for students of all sections of society. We believe in empowering every single student who couldn't dream of a good career in engineering and medical field earlier.
Our Key Focus Areas
Physics Wallah's main focus is to make the learning experience as economical as possible for all students. With our affordable courses like Lakshya, Udaan and Arjuna and many others, we have been able to provide a platform for lakhs of aspirants. From providing Chemistry, Maths, Physics formula to giving e-books of eminent authors like RD Sharma, RS Aggarwal and Lakhmir Singh, PW focuses on every single student's need for preparation.
What Makes Us Different
Physics Wallah strives to develop a comprehensive pedagogical structure for students, where they get a state-of-the-art learning experience with study material and resources. Apart from catering students preparing for JEE Mains and NEET, PW also provides study material for each state board like Uttar Pradesh, Bihar, and others

Copyright © 2025 Physicswallah Limited All rights reserved.