Monoclonal Antibodies And Hybridoma Technique

Monoclonal antibodies and hybridoma technique

Milstein and Kohler (1972) developed this technique to prepare the monoclonal antibodies through rDNA and hybridoma cells.
By rDNA technique antigenic polypeptides are produced through transgenic organism, which is used as vaccines,

e.g., for hepatitis B, vaccine has been produced from transgenic yeast. Such antibodies are highly pure and specific without any unwanted part hence it has side effect or otherwise interruptive effect. It is, therefore,
referred as ‘magic bullets’. In passive immunity such antibodies provide full proof treatment of the disease.

Lymphoid Organs

In these organs maturation and proliferation of lymphocytes take place. These are of two types : Primary and Secondary lymphoid organs.
In Primary lymphoid organs maturation of lymphocytes takes place and lymphocytes (B and T cells ) acquire their antigen specific receptors,
e.g., bone marrow for B-cell and thymus for T-cell.
In Secondary lymphoid organs these matured lymphocytes proliferate and differentiate in response to their specific antigens. These are spleen, tonsils, lymph nodes (Peyer’s patches) . Hence acquired immune response usually develop here.